Over‐expression of secreted proteins from mammalian cell lines
نویسندگان
چکیده
منابع مشابه
Over-expression of secreted proteins from mammalian cell lines
Secreted mammalian proteins require the development of robust protein over-expression systems for crystallographic and biophysical studies of protein function. Due to complex disulfide bonds and distinct glycosylation patterns preventing folding and expression in prokaryotic expression hosts, many secreted proteins necessitate production in more complex eukaryotic expression systems. Here, we e...
متن کاملOverexpression of membrane proteins in mammalian cells for structural studies
The number of structures of integral membrane proteins from higher eukaryotes is steadily increasing due to a number of innovative protein engineering and crystallization strategies devised over the last few years. However, it is sobering to reflect that these structures represent only a tiny proportion of the total number of membrane proteins encoded by a mammalian genome. In addition, the str...
متن کاملSecreted Chemicals From Probiotic Bacteria Potentiate Th1 Pattern of Immune Cells and Apoptosis Induction in Breast Cancer and Gastric Adenocarcinoma Cell Lines
Cancer is one of the main causes of mortality. Therapeutic importance in some diseases, especially cancers has led to considering the anti-cancer effects of probiotics more than ever. Probiotics have stimulatory effects on the immunity and suppressor effects on cancer activity. Various studies report that probiotic bacteria have cytotoxicity effects on different types of solid tumors. In this r...
متن کاملMammalian cell overexpression and siRNA depletion
Yip3 was cloned into the mammalian vector pcDNA3.1. HEK293 cells were transfected with pcDNA3.1 human Yip3 or pcDNA3.1, collected 42 h later, lysed by osmotic shock and shearing, and membranes were purified by ultracentrifugation. Membrane recruitment was as published in buffer plus 100 mM (NH4)2SO4, 100 mM GTPgS, 0.1 mg ml 21 BSA, protease inhibitor cocktail (0.34 units ml aprotinin, 0.01 mg m...
متن کاملAnalysis of secreted proteins from Aspergillus flavus.
MS/MS techniques in proteomics make possible the identification of proteins from organisms with little or no genome sequence information available. Peptide sequences are obtained from tandem mass spectra by matching peptide mass and fragmentation information to protein sequence information from related organisms, including unannotated genome sequence data. This peptide identification data can t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Protein Science
سال: 2014
ISSN: 0961-8368,1469-896X
DOI: 10.1002/pro.2439